ansB [2019-07-04 13:55:29]

L-aspartase

locus: BSU23570

pI: 5.69

mw: 52.39 kDa

protein length: 475 aa

gene length: 1428 bp

function: aspartate degradation

product: L-aspartase

essential: no

ec: 4.3.1.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.6|Utilization of asparagine/ aspartate]

Gene

Coordinates: 2,454,347 2,455,774

The protein

Catalyzed reaction/ biological activity

• L-aspartate = fumarate + NH₃ (according to Swiss-Prot)

Protein family

• class-II fumarase/aspartase family (with [protein|search|fumc], according to UniProt)

Paralogous protein(s)

• [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|CitG]

Structure

• [PDB|3R6Q] (from ''Bacillus'' sp. YM55-1, 72% identity, 92% similarity) [Pubmed|21661762]

Expression and Regulation

Operons

• genes: [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
  description: [Pubmed|1711029]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1711029], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|AnsR]: repression, [Pubmed|11914346], in [regulon|146947C974D62BCB282BA3F5B924B35695D75886|AnsR regulon]

  regulation

  • expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]

• genes: [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
  description: [Pubmed|22383849]

  regulation

  • ''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]

Biological materials

Mutant

• GP1153 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''ermC'') available in [SW|Jörg Stülke]'s lab
• BKE23570 ([gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
• BKK23570 ([gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA

lacZ fusion

• pGP2274 (in [SW|pAC5]) (GP2961), available in [SW|Jörg Stülke]'s lab

References

• 8478318,1711029,1711029,11914346,21219666,22383849